At the end of this course students will be able to: 1) Describe the fundamental principles of Newton's Laws and Conservation of Energy; 2) Describe basic interactions between charged particles and electric fields; 3) Define electric potentials; 4) Describe the relationship between electric and magnetic fields; 5) Identify the direction a charged particle will move in a magnetic field; and 6) Derive the relationship between charge, mass and velocity

At the end of this course students will be able to: 1) Describe the composition of typical biological collections including blood and waste; 2) Describe best-practices for preparing collections for mass spectrometry; 3) Describe fundamentals of separating biomolecules according to size, charge and other physiochemical factors; and 4) Describe mechanisms and tradeoffs behind common approaches for separating biological samples via chromatography (liquid and gas)

At the end of this course students will be able to: 1) Describe the basic technologies and instrumentation currently used for mass spectrometry; 2) Understand the basics in how to operate this instrumentation (theory, no hands on); 3) Appreciate the similarities and differences in the different technologies, and in what situation each instrument would be most appropriate; and 4) Describe the instrumentation used in sample preparation

At the end of this course students will be able to: 1) Describe key approaches for matching MS spectra to known proteins, as well as identifying and quantifying protein content in analyzes samples; 2) Describe methodologies and limitations thereof for labeling and quantifying proteins of interest; 3) Perform routine computational analyses of MS data using python; and 4) Describe best-practices for objective data analysis and reproducibility

At the end of this course students will be able to: 1) Compare and contrast aspects of good vs bad biomarkers and the utility of mass spectrometry for their detection in biological samples; 2) Describe best practices for sample collection and the limitations of using biological samples; 3) Describe current regulations and standards for biomarker use in the clinic; 4) Identify and describe biohazard safety issues when working with biological samples; 5) Describe analytical approaches for quantification of biomarkers; and 6) Describe the type of mass spectrometry assays typically used in a clinical or hospital setting

At the end of this course students will be able to: 1) Understand the need for specialized mass spec approaches; 2) Appreciate the theory behind specialized mass spec approaches; 3) Describe the technical and practical considerations when utilizing specialized MS approaches; and 4) Be able to choose the correct advanced MS approach for a particular situation

At the end of this course students will be able to: 1) Process liquid and tissue biological samples for analysis by mass spectrometry; 2) Perform gel electrophoresis on prepared samples and excise selected gel bands; 3) Perform in in-gel digestions using standard enzymatic approaches; 4) Perform in-solution digestions using standard enzymatic approaches; 5) Quantify protein samples before and after digestion; and 6) Use column chromatography to generate high purity samples

At the end of this course students will be able to: 1) Setup HPLC, mass spectrometer and software; 2) Perform normal instrument upkeep including calibration, quality control, solution changes; 3) Be able to troubleshoot common instrument issues; 4) Analyze sample on mass spectrometer for discovery (two group comparison); 5) Analyze sample on mass spectrometer for targeted quantification (in simple solution); 6) Use bioinformatics software packages to analyze data from mass spectrometers; and 7) Understand how to present mass spectrometry data

At the end of this course students will be able to: 1) Design quantitative experiments using mass spectrometry; 2) Describe and identify appropriate experimental positive and negative controls; 3) Describe statistical analyses relevant for their experimental design; 4) Discuss limitations and pitfalls of their research approach and describe alternatives; and 5) Critically evaluate the current literature and describe the rationale for their research study

At the end of this course students will be able to: 1) Design quantitative experiments using mass spectrometry; 2) Describe and identify appropriate experimental positive and negative controls; 3) Describe statistical analyses relevant for their experimental design; 4) Discuss limitations and pitfalls of their research approach and describe alternatives; and 5) Critically evaluate the current literature and describe the rationale for their research study